2,4-Dichlorophenoxyacetic Acid Inhibits the Outer Membrane NADH Dehydrogenase of Plant Mitochondria

The internal alternative NADH dehydrogenase of Neurospora crassa mitochondria.

An open reading frame homologous with genes of non-proton-pumping NADH dehydrogenases was identified in the genome of Neurospora crassa. The 57 kDa NADH:ubiquinone oxidoreductase acts as internal (alternative) respiratory NADH dehydrogenase (NDI1) in the fungal mitochondria. The precursor polypeptide includes a pre-sequence of 31 amino acids, and the mature enzyme comprises one FAD molecule as ...

Response of Oxidation & Coupled Phosphorylation in Plant Mitochondria to 2,4-Dichlorophenoxyacetic Acid.

The herbicidal actioIn of 2,4-dichlorophenoxyacetic acid (2,4-D) has been the subject of many investigations in the years sinice this compound was first demonstrated to be a plant toxicant. Almost as many possible mechanismiis for this toxicityhave been suggested. These includle depletioni of respiratory substrate, abnormal cellular proliferation, the production of toxic substances in response ...

The Preprotein Translocation Channel of the Outer Membrane of Mitochondria

The protein translocase of the outer mitochondrial membrane (TOM) serves as the main entry site for virtually all mitochondrial proteins. Like many other protein translocases it also has an ion channel activity that can be used to study the dynamical properties of this supramolecular complex. We have purified TOM core complex and Tom40, the main pore forming subunit, from mitochondria of the fi...

Membrane proteins in the outer mebrane of plastids and mitochondria

Generation of superoxide anion by the NADH dehydrogenase of bovine heart mitochondria.

Submitochondrial particles from bovine heart in which NADH dehydrogenase is reduced by either addition of NADH and rotenone or by reversed electron transfer generate 0.9 +/- 0.1 nmol of O2-/min per mg of protein at pH 7.4 and at 30 degrees C. When NADH is used as substrate, rotenone, antimycin and cyanide increase O2- production. In NADH- and antimycin-supplemented submitochondrial particles, r...